COGEM advice clarifying the proper use of the ‘COGEM formula’ is now available in English

COGEM has published an English translation of her advice on the explanation of the use of the ‘COGEM formula’. This formula can be used to calculate how many infectious lentiviral or retroviral vector particles (free viral particles) remain after transduction of cells outside the body (ex vivo transduced cells) in the product. The formula is used in both laboratory research and clinical gene therapy studies. In 2020, several parameters from the formula were experimentally validated and the COGEM formula was adjusted. Because it was noticed that the COGEM formula is not always applied correctly, an advisory report was issued to provide more explanation of the variables used and the influence that different experimental conditions and vectors have on the variables used. Although experimental determination of the number of remaining vector particles provides the most accurate approximation, the COGEM formula offers the best alternative for determining the number of remaining vector particles. Read the advisory report here: Advice explanation of the COGEM formula for calculating residual lentiviral or gammaretroviral vector particles